Multiplexed electrodes

Figure 1. Schematic diagram for digital noise measurements using multiplexed electrodes.

MesoScale Discovery (MSD) succeeded in introducing product with a similar technology approach based upon ruthenium redox-mediated electrochemical detection (Figure 2.14). MSD is a joint venture of its parent company, MesoScale, and IGEN, a company that pioneered much of fhe work on electrochemical detechon based on the ruthenium redox system. MSD s Multi-Spot plates contain antibodies immobilized on multiple working electrode pads within each well, allowing each spot within the well to serve as an individual assay. Multiplexed cytokine immxmoassays can be performed in 96-well (4,7, or 10 spots per well) patterns with detection limits of 1 to 10 pg/mL and a linear dynamic range up to 3,000 pg/mL. Both 24-and 384-well electrode systems are available. 

Multiplex systems have an array of electrodes on one of the substrates that addresses the rows, and another set on the other substrate that addresses the columns. 

It is more than probable that arrays for 50-100 DNA sequences will be needed for some clinical applications. Although it is not difficult to design electrode pads with reproducible dimensions of a micron or less, the electrochemical readout requires mechanical connections to each individual electrode. Therefore, the construction of very large, multiplexed arrays presents a major engineering challenge. Electronic switches in the form of an on-chip electronic multiplexer may provide a possible solution for this problem. 

Direct, multiplex and active matrix addressing are the three electronic drive methods used to generate the appropriate voltage at a particular pixel of an LCD, see Figures 2.11-2.14. The size, shape and pattern of electrodes on LCD substrates are fashioned to be compatible with the chosen method of addressing. In directly addressed LCDs the desired pattern of pixel electrodes is created by etching on one surface. A non-pattemed back electrode on the second surface provides the electrical contact. LCDs with multiplexed addres-... 

Figure 2.14 Schematic representation of the pixels, made up of a pattern of orthogonal rows and columns of electrodes, of an LCD with multiplexed addressing.

Multiplex addressing with M electrode columns and N electrode rows allows M X N pixels to be created driven by M + N connections made at the end of each row and column, see Figure 2.14. This type of addressing allows large-area, high-information-content LCDs to be produced with acceptable contrast and viewing angles, see Chapter 3. 

OLEDs can be addressed in a similar fashion to LCDs, see Chapter 2, i.e. directly with segmented electrodes, see Figure 4.8, by multiplexed addressing with rows and columns of electrode strips, see Figure 4.9 and by active matrix addressing with one transistor at each pixel, see Figure 4.10. The major... 

---