Multiple quantum coherence filtration

By allowing multiple-quantum coherence to process during the evolution period of a two-dimensional experiment, Drobny et al. were able to detect its effects indirectly. This idea subsequently blossomed into the new technique of filtration through double-quantum coherence. Multiple-quantum coherence of order n possesses an n-fold sensitivity to radiofrequency phase shifts, which permits separation from the normal single-quantum coherence. This concept inspired the popular new techniques of double-quantum filtered correlation spectroscopy (DQ-COSY) and the carbon-carbon backbone experiment (INADEQUATE), both designed to extract useful connectivity information from undesirable interfering signals. 

Phase cycling As employed in modern NMR experiments, repeating the pulse sequence with all the other parameters being kept constant and only the phases of the pulse (s) and the phase-sensitive detector reference being changed. The FIDs are acquired and coadded. The procedure is used to eliminate undesired coherences or artifact signals, or to produce certain desired effects (e.g., multiple-quantum filtration). 

The first of these cycles also selects Ap = 6 for the first two pulses i.e. filtration through six-quantum coherence normally, we can safely ignore the possibility of such high-order coherences. The second of the cycles also selects Ap = +5 and Ap = -7 on the last pulse again, these transfers involve such high orders of multiple quantum that they can be ignored. 

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