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Multimodal nonlinear microscopy

The chemical specificity of CARS microscopy is readily combined with other nonlinear optical image contrast mechanisms, such as two-photon fluorescence (TPF), SHG, and THG, resulting in a multimodal CARS microscopy [88, 118, 117, 43]. In multimodal nonlinear optical imaging, TPF, SHG, and THG signals all benefit from the use of femtosecond laser pulses of high peak intensities, whereas the contrast and chemical selectivity of CARS benefits from the use of picosecond (narrow-bandwidth) pulses (see discussion in Sect. 6.2.3). As demonstrated by Pegoraro et al. [43], this apparent... [Pg.128]

Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and nonlinear Raman microspectroscopy. The text explores the promise of beam shaping and the use of a broadband laser pulse generated through continuum generation and an optical pulse shaper. [Pg.279]


See other pages where Multimodal nonlinear microscopy is mentioned: [Pg.568]    [Pg.569]    [Pg.571]    [Pg.568]    [Pg.569]    [Pg.571]    [Pg.98]    [Pg.128]    [Pg.145]    [Pg.573]    [Pg.576]   


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