Multicontrast nonlinear microscope

Figure 4.6 shows a dark adapted, freshly isolated chloroplast imaged with a multicontrast nonlinear microscope coupled to Yb KGW laser radiating at 1030 nm (Major et al. 2006). The Yb KGW laser preferentially excites carotenoids via the... 

This chapter introduces principles of three nonlinear contrast mechanisms— MPF, SHG, and THG—for microscopic imaging. The instrumentation of nonlinear multicontrast microscopy is described, and the methods of multicontrast image analysis are presented. The later parts of the chapter focus on several examples of multicontrast nonlinear microscopy applications in biological imaging. 

FIGURE 4.1 The setup of the nonlinear multicontrast microscope. Microscope components are defined in the figure legend. See text for detailed description of the setup. 

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