Multi glycoconjugate synthesis

In order to avoid multi-step chemical syntheses to achieve regio- and stereospecific reactions, glycosidases have been employed as catalysts for glycoconjugate synthesis. Glycosidases are attractive for large-scale application since they are abundant catalysts, inexpensive, commercially available, and exhibit broad processing specificity, particularly with respect to the acceptor, as compared with more substrate specific glycosyltranferases [1]. 

Figure 5.1 General approach for the synthesis of glycoconjugates by multi-enzyme systems.

In the following sections we summarize multi-enzyme systems for the synthesis of glycoconjugates (Table 5.1) following the general scheme for the combination of enzyme modules (see Figure 5.1). Many of these multi-enzyme systems have been already reviewed - here we will highlight those which have been recently been developed. 

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