Mucosal reconstruction

Zalis ME, Perumpillichira J, Del Frate C et al. (2003) CT colonography digital subtraction bowel cleansing with mucosal reconstruction - initial observations. Radiology 226 911-917... 

Moreover, digital cleansing may introduce artifacts because of the partial volume effect and a sub-optimal mucosal reconstruction method, especially... 

Kinoshita S, Koizumi N, Nakamura T. Transplantable cultivated mucosal epithelial sheet for ocular surface reconstruction. Exp Eye Res 78 483 191 (2004). 

Of the different types of oral mucosal cell cultures that have been used [47,48], the most commonly used ones are explants of primary cultures. Small pieces of excised buccal or sublingual tissue are placed in a support system and fed with culture medium. The outgrowths obtained from these tissue explants are then transferred and grown in appropriate media. For example, outgrowths of fibroblasts [49] thus obtained have been described. Gibbs and Ponec [50] reconstructed the epithelium of mucosal tissue by placing a tissue biopsy (with the epithelial side upwards) onto a fibroblast-populated collagen gel. The explants obtained were cultured immediately at the air liquid interface until the epithelium had expanded over the gel (2-3 weeks). These explant cultures may retain many of the in vivo tissue characteristics. 

Nishida, K., Yamato, M., Hayashida, Y, Watanabe, K., Yamamoto, K., Adachi, E., et al. (2004b). Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. New England Journal of Medicine, 351, 1187-1196. 

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