MS-based lipidomics

D1 (10,17S-docosatriene) from DHA using tandem liquid chromatography-photodiode array-electrospray ionization-tandem mass spectrometry (LC-PDA-ESI-MS-MS)-based lipidomic analysis have been documented in ischemic brain [4] and retinal pigment epithelium [5], This new lipid is called neuroprotectin D1 (1) because of its neuro-protectiveproperties in brain ischemia-reperfusion [4] and in oxidative stress-challenged retinal pigment epithelial cells [5] (2) because of its potent ability to inactivate proapoptotic signaling (see apoptosis, Ch. 35) [5] and (3) because it is the first identified neuroprotective mediator derived from DHA. 

The novel approaches in LC-MS-based lipidomics, such as ultra-high performance LC (UHPLC), combined with high-resolution MS methodologies allow fast and reliable analysis of various classes of lipids (33). The modem instruments, with fast duty cycles, allow acquisition of high and low collision energy data simultaneously to provide both data on precursor ions and fragmentation data for all detectable molecular ions (34). 

Several new methods were developed including both ELISA and liquid chromatography-tandem mass spectrometry (LC/MS/MS)-based lipidomics for... 

Ions are generated in an electrospray ion source after a stream of lipid solution is pushed into the ion source chamber by a mechanical force (see Chapter 2). Depending on whether lipid analysis conducted with the lipid solution delivered to the ion source chamber is under a constant lipid concentration condition, these ESI-MS-based lipidomics approaches can be classified into two major categories ... 

As the development of lipidomics field, mass spectrometric analysis of plant lipids has been advanced accordingly. The branch of lipidomics in analysis of plant cellular lipids utilizing mass spectrometry was specifically called plant lipidomics [1], As described early in the book (Chapter 3), all kinds of MS-based lipidomics approaches, particularly those based on ESl-MS or ESI-MS/MS, could be used in plant lipidomics. The advantages of ESl-MS-based methods over traditional lipid analytical approaches for analysis of plant lipids were extensively discussed [1-3]. 

Due to its novelty, MS-based lipidomics is still evolving and relative few practical applications have emerged so far. However, it is highly likely that in the near future MS-lipidomics will play, in combination with other omics, a crucial role in biology, biotechnology, and medicine. 

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