1.4- MPB

The synthesis of TNU-9 in the presence of 1,4-MPB was found to be possible only from synthesis mixtures with a very narrow range of Si02/ AI2O3 and Na0H/Si02 ratios, which is also the case of the 1,5-MPP-mediated synthesis of mainly due to the flexible nature of the... 

Table 5.3 Representative products obtained using l,4-bis(N-methylpyrrolidinium)-butane (1,4-MPB) as an OSD A. Reprinted with permission from S.B. Hong, Catal. Surv. Asia, Synthesis of all-silica molecular sieves in fluoride media. Vol 12, 131. Copyright (2008) Springer...

Table 5.3 Representative products obtained using l,4-bis(A-methyl-pyrrolidinium) butane (1,4-MPB) as an organic SDA. ...

Block 1.5 mL (per selection) streptavidin M-280 Dynabeads by adding 5 mL 4% MPBS, and rotate in a 15-mL tube on an end-over-end mixer for 5 min at room temperature... 

Pour off the supernatant, and add another 5 mL 4% MPBS. Rotate for 1 h at room temperature, and separate again with a magnetic particle concentrator... 

Repeat the selection procedure another three to four times (use a 1 -mL aliquot for selection, and store the remaining phage at 4°C). For the second and subsequent rounds of selection, mix 1 mL phage from the previous round of selection with 0.5 mL of 6% MPBS, 10 pL Tween-20, and biotinylated antigen to give a final concentration of 50 nM Use only 300 pL of blocked streptavidin-coated Dynabeads... 

The kinetic procedure used for calculation of C from N-data (Sect. 4.1.2.1) can be modified to yield relevant equations applied when MPB-data are available. Thus, if Rp and Rtl are time-independent, then equations ... 

Prepare liposomes incorporating 1% /V-(4-(p-maleidophcnyl)butyryl)-l,2-.vn-distearoylphosphatidylethanolamine (MPB-DSPE) as described above. This should be done not more than 1 d prior to use. 

The initial PIA purification method was developed by Mack et al. (3). These authors used a different, two-step chromatography protocol involving size-exclusion and ion exchange chromatography on Sephadex G-200, Q-Sepharose, and S-Sepharose. A similar purification method has been described recently to isolate a PIA-related polysaccharide polymer in E. coli (7). Briefly, E. coli cells were incubated in 50 raM Tris-HCL buffer (pH 8.0), 100 mg lysozyme, and 0.1 M EDTA at room temperature for 2 h. Phenol/chloroform extraction steps were performed to separate protein and debris contamination from the polysaccharide. Samples were concentrated by ultrafiltration devices (10,000 MW cut off) and fractionated on a fast protein liquid chromatography (FPLC) system with a Sephacryl S-2000 column (equilibration and elution buffer 0.1 MPBS, pH 7.4). 

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