Morphology and Subcellular Organization of Cells

Figu re 5.9 (a) Brightfield image of a HeLa cell, attached to a Cap2 window, in buffer. Scale bar= 10 im (b) Raman spectral image, obtained via hierarchical cluster analysis (HCA) from a hyperspectral data cube collected atSOOnm spatial resolution (488 nm 

These results demonstrate that confocal Raman microspectroscopy, when coupled to image reconstruction methods such as HCA, can provide label-free methods for the visuahzation of intracellular components and processes. This subject will be elaborated further in the next section. 

---