Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

MORIN-fsDNA

FLUORESCENCE ENHANCEMENT OF KI FOR THE MORIN-fsDNA SYSTEM AND ITS ANALYTICAL APPLICATION... [Pg.377]

There were two possible explanations for the fluorescence enhancement of morin-fsDNA upon the addition of KI. [Pg.377]

Fluorescence Enhancement ofKlfor the Morin-fsDNA System 379... [Pg.379]

The first reason for the enhancement effect was the formation of the more favorable structure for luminescence. The studies on the effect of different halogen anion compounds (KC1, KBr and KI) on the morin-hsDNA systems were conducted. The results showed that at the same concentrations and conditions (<1.0x10 3mol/L), KCI weakened the fluorescent intensity of the system, while both KBr and KI enhanced it, and the enhancement order was KI > KBr. It is proposed that the lone electron pair of electronegative I (or Br ), bonds to the conjugated system of morin, and that the combined system then interacted with fsDNA. With the increase of atomic radius from Br to I, the attraction between nucleus and outer electrons of... [Pg.377]

We also studied the binding properties of KI-morin-hsDNA system by absorption, circular dichroism and the ionic strength effect experiments. The studies indicated that the groove binding mode was dominant in the KI-morin-DNA system. Fluorescence titration result revealed that Kl-morin showed a good linear response to fsDNA. The calibration curves for the determination of hsDNA and smDNA were constructed under the optimal conditions. All the analytical parameters are presented in Table 1. [Pg.378]


See other pages where MORIN-fsDNA is mentioned: [Pg.378]    [Pg.378]   
See also in sourсe #XX -- [ Pg.381 ]




SEARCH



Morine

© 2024 chempedia.info