Monosaccharides weight ratios

From the carbohydrate analyses of peak II, it was determined that 780 pg of polysaccharide (i.e., polymers of neutral monosaccharides with polymer mol. wt. of 1,000-5,000) were reversibly complexed with the estimated 19.5 pg of glycopeptide contained in this peak. Hence, the calculated carbohydrate glyco-peptide weight ratio is approximately 40 1. 

Maximum labelling of heparin with F-D was achieved at 5 hours at 25 °C, pH 8.4. In the case of heparin, the efficiency of labelling was not dependent on molecular weight, but solely a function of the ratio of the concentrations of labelling reagent to monosaccharide subunit in the reaction mixture. Similar results were encountered in the labelling of dextrans of different molecular weight (9). 

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