Modulation of Structural Joint Integrity Revealed by Histological Scoring

Modulation of Structural Joint Integrity Revealed by Histological Scoring [Pg.252]

Tissue Processing Skinned mouse knee joints are immersed in neutral buffered formaldehyde (3.7 %) directly after preparation. Fixation times are limited to one week to ensure tissue integrity. Tissue samples are rinsed overnight with tap water. Tissue samples that contain bone are decalcified (D-calciher normal, Shandon 1779) for 2 or 3 days. Decalcified samples or cartilage samples without bone are rinsed overnight with tap water. After dehydration with alcohol in stepwise increasing concentrations, tissue samples are embedded in paraffin. [Pg.253]

Although the Mankin score is still widely used to assess histopathological changes semiquantitatively, the group of Salter remarked on its limitations with respect [Pg.253]

Instead of a more elaborate score, Brewster et al. (1998) reduced the staging originating from the Mankin grades to a more simplified score, which still leads to statistically significant differences between the treatment groups. [Pg.254]

The group of van den Berg prefers for assessment of structural changes in the mouse knee the coronar sections over the sagittal plane (van der Kraan et al. 1994), depicting both condyles, and facilitating the detection of osteophytes. [Pg.254]

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