Modified Ussing chamber

As shown in Figure 4.2, the modified Ussing chamber system consists of two half chambers which when clamped together are separated by a piece of... 

Figure 4.2 The modified Ussing chamber consisting of a donor and a receptor chamber which when clamped together are separated by a piece of tissue (intestinal, nasal, buccal, etc). The available area for diffusion (highlighted in a green circle to enhance visibility) is 0.64 cm2 and the volume of solution applied to each chamber is 1.5 ml. The applied solution is bubbled with carbogen gas (95% O2 + 5% CO2) that enters the chambers through the gas ports (See also Color Insert.)...

Manipulating cychc guanosine monophosphate levels by sodium nitroprusside (10 M), atrial natriuretic peptide (10 M), brain natriuretic peptide (10 M), and C-type natriuretic peptide (10 M) aU had no effect on short-circuit current measurements when added both the serosal and mucosal surfaces of ovine tracheal epithelia mounted in modified Ussing chambers (Range et al. 1997). 10 M 3-Isobutyl-1-methylxanthine (a non-specific phosphodiesterase inhibitor) when added to the mucosal surface resulted in a mean reduction in short-circuit current of 4.3 [lA. cm" (8.9 % of baseline). 

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