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Modified side chains, elucidation

Proteins for pharmaceutical use should not have modified side chains. The common method for detection of modified residues is to see a blank space instead of a peak in the HPLC elution trace of the amino acid analysis or a band at another position than the amino acid indicated by the gene sequence. This analysis will probably not detect modifications that affect only a small fraction of the protein. Elucidation of the identity of the modified side chains, if one can locate them, requires some astute chemistry. For example, in phycocyanine, N-methyl-asparagine ran exactly at the position of serine Identification of the modified residue relied on detection of methyl amine released by acid hydrolysis of the protein. ... [Pg.30]

In an effort to elucidate the structural requirements for biological activity of vitamin D, several groups have modified the size and/or substitution pattern of the side chain. [Pg.85]

See other pages where Modified side chains, elucidation is mentioned: [Pg.625]    [Pg.33]    [Pg.366]    [Pg.204]    [Pg.1608]    [Pg.158]    [Pg.66]    [Pg.83]    [Pg.1090]    [Pg.385]    [Pg.436]    [Pg.98]    [Pg.210]    [Pg.468]    [Pg.141]    [Pg.46]    [Pg.391]    [Pg.668]    [Pg.272]    [Pg.225]    [Pg.129]    [Pg.499]    [Pg.110]    [Pg.129]   
See also in sourсe #XX -- [ Pg.30 ]



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Elucidation

Side-chain-modified

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