Minimal inhibition concentrations

Analyzing the minimal inhibition concentration (MIC) data for nine different strains, resulted in two significant PCs being obtained, accounting for 77.1% and 16.1%, respectively, of the data variance. Thus, the intrinsic dimensionality of the data matrix of nine assays was only two. The loading plot, that is, a plot of the calculated PCs with respect to the descriptors, shows that the first component is mainly related to the seven cell-free test systems, while the second component represents the two whole-cell test results. Thus, there was much correlation between the results of the seven cell-free assays and the two whole-cell assays were correlated with each other but not with the cell-free assays. In other words, much redundant information was obtained... 

Another common method is the incorporation of materials into cooled agar or broth either in the presence or absence of a solvent. Without a solvent, the poor solubility of the essential oil becomes a significant issue. To solve this problem, solvents or detergents are typically used. Tween and ethanol are common solvents for lipophilic materials, such as essential oils, to aid dissolution into an aqueous environment. Unfortunately, many of these materials affect activity. For example, when thyme oil or thymol (91) is tested in the presence of Tween 80, its activity is markedly decreased when compared with a system without Tween [114]. Furthermore, minimal inhibition concentrations (MICs) and minimal lethal... 

Table 6.22. Antibacterial activity in vitro of nitrofurans minimal inhibiting concentrations /iglm. ), 24 hours...

Test Method. A minimal inhibition concentration (MIC) method, as described in NCCLS-M7, is used. 

Sadler, M. and Binder, J. (1996) Determination of the minimal inhibiting concentration of different substances and substance mixtures with the aid of a row-dilution-test. IFA Tulin, In-house report 1-5. 

The first step in a screening cascade is normally the determination of the minimal inhibition concentrations against bacteria, fungi or algae. 

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