Mini-sequencing

Pastinen T, Kurg A, Metspalu A, Peltonen L, Syvanen AC. Mini-sequencing a specific tool for DNA analysis and diagnostics on oligonucleotide arrays. Gen Res 1997 7 606-614. 

Liu, Y., Sun, X., Guo, B. (2003) Matrix-assisted laser desorption/ionization time-of-flight analysis of low-concentration oligonucleotides and mini-sequencing products. Rapid Commun Mass Spectrom, 17, 2354-2360. 

Fig. I Genotyping method by mini-sequencing (see text). Each step from DNA purification to hybridization and detection takes place in separate chambers on a disposable chip...

Figure 3.21 Schematic of the fabrication sequence for the mini packed-bed reactor [80],...

Consideration of the relationship between the effects of radiation on homopolymers and copolymers raises the question of the variation from homopolymer behaviour with sequence length. Every copolymer has a distribution of sequence lengths for each comonomer. At what minimum sequence length does methyl methacrylate not show the high scission of PMMA The future will probably see the development of processes for making polymers with controlled mini-block sequences to maximize a number of properties such as scission yield, adhesion, flexural strength, Tg.. 

In addition to incorporating the 4-(2-aminoethyl)dibenzofuran-6-propanoic acid template into small peptides where a reverse turn is desired, we have also recently incorporated this template into a mini-protein called the PIN WW domain. WW domains have a three-stranded antiparallel p-sheet structure that mediates intracellular protein-protein interactions. 31 Substitution of this 3-turn mimetic into loop 1 of the PIN WW domain leads to a folded, three-stranded, antiparallel p-sheet structure with a stability indistinguishable from that of the all a-amino acid sequence. The template-incorporated PIN WW domain (11) was synthesized by an Fmoc-based solid-phase peptide synthesis strategy (Scheme 8), utilizing N-Fmoc-protected 4-(2-aminoethyl)dibenzofuran-6-propanoic acid 10. 11 The synthesis of 10, similar to that of 8, has been published.1 1 ... 

The copolymer generator calculates a series of "mini-batch" copolymerizations and has been described in more detail by Molau (19) and Meyer and Lowry (20). The sequence distribution generator uses Harwood s (14) run number approach to calculate triad functions. 

Another alternative is the insertion of small sequences of human chromosomes into embryonic animal cells, thus generating trans-chromo-somic mice. These mini chromosomes are isolated from human chromosomes 2 and 14, which contain the genes for the light and heavy chains, respectively. This means that all of the V, D, and J segments of the variable N-terminal portion, as well as those of the constant regions, will become part of the mouse genome (van Dijk and van de Winkel, 2001 Roque et al., 2004). 

The TMA map may consist of a simple Excel sheet or may be a more sophisticated datasheet made using one of the TMA generation programs. This will serve as a guideline to in order arrange blocks and sequence in which they need to be arrayed. Thus the TMA map will contain the exact location of each case, including the duplicate samples, and controls are located. Mini-arrays ( City Blocks ) of the cores (3x5, 4x5, 5x5, 6x5) can be spaced for easy orientation, with control tissue in the rows between the mini-arrays. 

The products from the translation of antisense RNA. Some antisense peptides have been demonstrated to show affinity properties that appear to be unique to that sequence and not seen in scrambled sequences. See Schwabe, C., New thoughts on the evolution of hormone-receptor systems, Comp. Biochem. Physiol. A 97, 101-106, 1990 Chaiken, I., Interactions and uses of anti-sense peptides in affinity technology, J. Chromatog. 597, 29-36, 1992 Labrou, N. and Clonis, Y.D., The affinity technology in downstream processing, J. Biotechnol. 36, 95-119, 1994 Root-Bernstein, R.S. and Holsworth, D.D., Antisense peptides critical mini-review, J. Theoret. Biol. 190, 107-119, 1998 Siemion, I.Z., Cebrat, M., and Kluczyk, A., The problem of amino acid complementarity and antisense peptides, Curr. Protein Pept. Sci. 5, 507-527, 2004. 

---