Microsatellite sequences

Zarlenga, D.S., Aschenbrenner, R.A. and Lichtenfels, J.R. (1996) Variations in microsatellite sequences provide evidence for population differences and multiple ribosomal gene repeats within Trichinella pseudospiralis. Journal of Parasitology 82, 534-538. 

Other genetic markers may be identified in this way, such as minisatellite and microsatellite sequences (see Qiapter 7). 

Paternity Testing Using PCR Amplification of Microsatellite Sequences... 

Edwards KJ, Barker JHA, Daly A, Jones C, Karp A (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20 758-760... 

Below is given a protocol for PCR amplification of microsatellite sequences using internal radiolabelling and a touchdown protocol which has been successfully used to analyze microsatellite instability in human, mouse and canine DNA. 

Shridhar, V., Siegfried, J., Hunt, J., del Mar Alonso, M., and Smith, D. I. (1994) Genetic instability of microsatellite sequences in many non-small cell lung carcinomas. Cancer Res. 54, 2084-2087. 

Chiaravalli AM, Furlan D, Facco C, et al. Immunohistochemical pattern of hMSH2/hMLHl in familial and sporadic colorectal, gastric, endometrial and ovarian carcinomas with instability in microsatellite sequences. Virchows Arch. 2001 438 39-48. 

Locus Microsatellite sequence Species Isolate Reference... 

In DNA, there are many classes of satellite and microsatellite sequences that consist of many copies of a simple repeat unit. 

Microsatellite repeat sequences Dispersed or group repeat sequences of 2-5 bp repeated up to 50 times. May occur at 50-100 thousand locations in the genome. 

Polymorphism A common (i.e., at least 1% prevalence of the minor allele in the population) sequence variation observed in an individual at a polymorphic site. Polymorphisms include nucleotide substitutions, insertions, deletions and microsatellites. They may be functional or silent, i.e., they do not result in detectable differences in gene expression or protein function. 

Another example is the study of microsatellites that are short DNA loci having simple sequence repeats that are widely distributed throughout the human genome. They are a valuable source for human genetic linkage analysis and molecular cancer research... 

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