Micropuncture Techniques in the Rat

Micropuncture techniques have been applied to the direct investigation of the effect of diuretics on single nephron function. The observed changes in tubular fluid reabsorptive rates and electrolyte concentrations can be used to asses the mechanism of action. The 

The following parameters may be determined inulin clearance (GFR), single nephron GFR, fractional delivery of water, sodium and potassium in proximal and distal tubules and in urine. All data are expressed as mean values SEM. Comparison of the effects of compounds to be tested with controls is performed by one way analysis of variance and by Student st-test for paired and unpaired data. 

Duarte CG, Chomety F, Giebisch G (1971) Effect of amiloride, ouabain and furosemide on distal tubular function in the rat. Am J Physiol 221 632-640 

Hropot M, Fowler N, Karlmark B, Giebisch G (1985) Tubular action of diuretics Distal effects on electrolyte transport and acidification. Kidney Int 28 477-489 

Knox FG, Marchand GR (1976) Study of renal action of diuretics by micropuncture techniques. In Martinez-Maldonado M (ed) Methods in Pharmacology, Vol 4A, Renal Pharmacology. Plenum Press, New York, London, pp 73-98 

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