Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Microinjection of fluorescent dyes

Saito, M. Ohi, A. Matsuoka, H. Microinjection of fluorescent dye in a plant cell and its intercellular translocation using a multichannel microelectrode system. Biochim. Biophys. Acta, Gen. Sub. 1996, 1289, l-Ar. [Pg.267]

Quality control methods for each protocol are also presented. Applications and representative data are presented for the sensor controlled aerosol and matrix microinjection, whereas representative data for matrix solution fixation are reported elsewhere (8). Sensor controlled aerosol is applied to multimodal cell tracking by fluorescence and multiple reaction monitoring (MS/MS) of a fluorescent dye incorporated into stem cells and tumor cells surgically implanted into a mouse brain. This protocol enables validation of MSI using confocal microscopy for the tracking of implanted cells, and potentially the effect of a given cell s microenvironment upon its molecular composition. Matrix microinjection is used to acquire a MALDI mass spectrum of a single motor neuron from layer V of a mouse motor cortex. [Pg.420]

A conceptually related technique is based on microinjection of a fluorescent tracer and on monitoring the expansion of the fluorescent plume by CSLM (De Beer et al. 1997). Instead of diffusion into a depleted area (FRAP), the out-diffusion is followed into the surrounding area. The distribution pattern of the dye is fitted with an implicit equation to obtain a local value for D, the diffusion coefficient. A refinement of this technique is to detect the fluorescence with an optical fiber connected to the microinjection capillary (De Beer et al. 1997 Kiihl and Revsbech 1998). This microsensor can be used in thick nontransparent hiofilm.s. With this technique,. similar ohservations were obtained as with FRAP the diffusion coefficient of small molecules (MW 300) is close to that in water, while the mohiKty of large molecules (MW 240,000) is decreased to ca. 30 %. [Pg.356]

Earlier techniques for measuring cytosolic free Ca2+ (1-2) such as the luminescent photoprotein aequorin, the absorbance dye arsenazo III and Ca2+-sensitive microelectrodes, all required microinjection or impalements, and were therefore applied mainly to giant cells. Later, photoproteins have been loaded with various reversible permeabilization procedures (3), but the largest expansion in the range of cell types in which Ca2+ signals can be quantified has come from the development of new fluorescent indicators that can be loaded using hydrolyzable esters. Currently four fluorescent indicators are used frequently quin-2, fura-2, indo-1 and fluo-3. [Pg.144]


See other pages where Microinjection of fluorescent dyes is mentioned: [Pg.17]    [Pg.350]    [Pg.17]    [Pg.350]    [Pg.297]    [Pg.235]    [Pg.216]    [Pg.439]    [Pg.65]    [Pg.66]    [Pg.123]    [Pg.200]    [Pg.741]    [Pg.161]    [Pg.23]    [Pg.599]    [Pg.556]    [Pg.1397]    [Pg.289]    [Pg.75]    [Pg.23]    [Pg.200]    [Pg.214]    [Pg.655]    [Pg.114]   
See also in sourсe #XX -- [ Pg.2 , Pg.40 ]




SEARCH



Fluorescence dye

Fluorescent dyes

Microinjection

Of dyes

Of fluorescent dyes

© 2024 chempedia.info