Microinjection drawbacks

Microinjection involves the direct injection of nucleic acids into the nucleus or cytoplasm of target cells and is the simplest approach for gene delivery. Thin glass capillaries are used to inject nanoliters of nucleic acid solution into cells. One major drawback of this method is obviously the throughput every single cell has to be manipulated individually. This limits the use of microinjection to applications in which individual cell manipulation is possible, such as genetic engineering of... 

To develop strategies that overcome the drawbacks of DNA microinjection, one has to consider the fate of injected DNA inside a cell. Plasmid DNA, injected... 

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