Microdissection, laser-assisted

Wellmann et al. (2002) also examined prostate carcinoma with the aid of laser-assisted microdissection and SELDI to analyze protein extracts from about 500 cells. A number of differentially expressed proteins in the 1.5- to 30-kDa range were found between normal prostate gland cells and prostate tumors. A prominent (threefold) up-regulated protein peak at mass 4299 Da was observed for prostate tumor cells. The average relative intensities were 24.37 for tumor cells, 9.99 for transitional zone cells, and 7.26 for normal prostate cells. 

Fend F, Raffeld M. Laser capture microdissection in pathology. J Clin Pathol 2000 53 666-672. Alevizos I, Mahadevappa M, Zhang X et al. Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis. Oncogene 2001 20 6196-6204. 

Pahner-Toy, D.E., Sarracino, D.A., Sgroi, D., LeVangie, R. and Leopold, P.E., Direct acquisition of matrix-assisted laser desorption/ionization time-of-flight mass spectra from laser capture microdissected tissues. Clin. Chem., 46, 1513-1516 (2000). 

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