Micrococcus aerogenes

The anaerobe Peptococcus (Micrococcus) aerogenes had a dehydrogenase that carried out specific hydroxylation at the 6-positions of 2- and 8-hydroxypurine, and was therefore distinct from xanthine dehydrogenase from which it could be separated (Woolfolk et al. 1970). It was also able to carry out dismutation of 2-hydroxypurine to xanthine (2,6-dihydroxypurine) and hypoxanthine (6-hydroxypurine). 

Woolfolk CA, BS Woolfolk, HR Whiteley (1970) 2-oxypurine dehydrogenase from Micrococcus aerogenes. I. 

Recently Jensen and co-workers have determined the structure of a clostridial-type ferredoxin obtained from Micrococcus aerogenes (47). One of the two apparently identical iron-sulfur clusters is illustrated in Fig. 2. The structure is compatible with a model with iron and labile sulfide at alternate comers of a cube. This accounts for the equivalence of these moieties in the protein. Another 8-iron-8 labile sulfur ferredoxin, from Clostridium acidiurici, similarly contains two independent iron-sulfur clusters per molecule (48). Strahs and Kraut (49) had earlier discovered... 

Arsenate 33 Desulfotomaculum auripigmentum Desulfovibrio strain Ben-RA V. atypica Micrococcus aerogene Newman et al. (1997b) Macy et al. (2000) Wolfolk andWhiteley (1962) Wolfolk andWhiteley (1962)... 

Clostridium pasteurianum Peptostreplococcus elsdenii Micrococcus aerogenes Desulfovibrio vulgaris ... 

Bachmayer, H., and K. T. Yasunobu The amino acid sequence of micrococcus aerogenes rubredoxin. Biochem. Biophys. Res. Commun. 26, 435 (1967). 

Clostridium pasteunanum Peptost reptococcus elsdenii Micrococcus aerogenes... 

Micrococcus aerogenes Brevibacterium Jlavum Bacillus licheniformis Clostridium SB4... 

Rubredoxin a redoxin, functionally similar to Fer-redoxin (see). M, 6,000. R. was isolat from Clostridium pasteurianum synthesis of R. appears to be promoted by a relative deficien<7 of iron. It contains one Fe atom/molecule of protein, which is less than the Fe content of ferredoxin. Under acid conditions it is more stable than ferredoxin, and it has a more positive redox potential (E o -0.057 V) thus when R. replaces ferredoxin in a feiredoxin-dependent reaction the reaction rate is decreased. The iron is bound by coordination with 4 cysteinyl residues other possible ligands are tyrosine and lysine. Redoxins similar to R. have been isolated from Peptostreptococcus elsde-nii and other baeteria. R. from Micrococcus aerogenes contains 53 amino acid residues of known sequence. 

Bacteria can make formate in many ways. A preliminary note by Chin et al. (1957) reports that the phosphoroclastic splitting of pyruvate to HCOOH -h CH3COOH by Escherichia coli was stimulated by FH4. Huen-nekens et al. (1958) refer to similar unpublished results (Whitely, 1958) with Micrococcus aerogenes and M, lactilyticus-, serine was not an intermediate. If the reaction HCOOH H2 + CO2, also carried out by E. coli, turns out to be folic-catalyzed, the presumption would be greatly strengthened that all formate-C02 reactions require FA, as might perhaps also formate-oxalate reactions. 

Plant aldolases are similar to mammalian aldolases, but have smaller molecular weights.347 The aldolases from Lactobacillus casei and Micrococcus aerogenes348 are similar to the mammalian enzymes with respect to Schiff-base formation349 and to involvement of specific amino acid residues.350 However, the amino acid composition of the enzyme from M. aerogenes is different from those of rabbit aldolases.351... 

B. subtilis, S. lutea, C. albicans P. aeruginosa and R. rubra E. coli, S. aureus, Listeria monocytogenes. Salmonella enteritidis Streptococcus oralis. Streptococcus anginosus. Streptococcus intermedius and Streptococcus sanguis, Enterobacter aerogenes. Micrococcus roseus. 

Activity as bactericide (1099) against Escherichia coli, Staphylococcus aureus (702), Bacillus subtilis. Micrococcus roseus (623), Aerobacter aerogenes, B. cereus var. mycoides (89 ). 

Activity as bactericide (9I, 622) against Aerobacter aerogenes (893) Achromo-bacter, Brevibacterium, Flavobacterium, Micrococcus, Pseudomonas, Sarcina (1275) intestinal bacillus (275) Staphylococcus aureus (91> 275) Bacillus proteus, Escherichia coli. Pseudomonas pyocyanea (11 5) ... 

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