Microchips, nucleic acid analysis

Vasiliskov VA, Prokopenko DV, Mirzabekov AD. Parallel multiplex thermodynamic analysis of coaxial base stacking in DNA duplexes by oligodeoxyribonucleotide microchips. Nucleic Acids Res. 2001 29 2303-2313. 

Radtkey R, Feng L, Muralhidar M, etal. Rapid, high-fidellty analysis of simple sequence repeats on an electronically active DNA microchip. Nucleic Acids Res 2000 28 E17 pp l-vl. 

The aim of this chapter is to cover the theoretical and practical aspects of capillary gel electrophoresis. It also provides an overview of the key application areas of nucleic acid, protein, and complex carbohydrate analysis, affinity-based methodologies, as well as related microseparation methods such as ultra-thin-layer gel electrophoresis and electric field-mediated separations on microchips. It also gives the reader a better understanding of how to utilize this technology, and determine which actual method will provide appropriate technical solutions to problems that may have be perceived as more fundamental. Micropreparative aspects and applications are discussed in Chapter 12. 

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