Microcarrier Culture and General Control Parameters

The method for animal cell culture is chosen according to whether the cell type is anchorage dependent or independent. For anchorage dependent cells, the cells must adhere to suitable material such as a plastic or glass dish or plate. As shown in Table 7, several types of culture methods were 

The most important factor in this method is the selection of a suitable microcarrier for the cells. Microcarriers are made of materials such as dextran, polyacrylamide, polystyrene cellulose, gelatin and glass. They are coated with collagen or the negative charge of dimethylaminoethyl, diethylaminopropyl and trimethyl-2-hydroxyaminopropyl groups as shown in Table 8, 

TYPE Nasi Supp tier Material s. c. Size ( i) S. A. (em/g) 

Tissue cu t ture treated Sips i ten Nunc. Foificrreee 1. 05 160-300 225 

Decrease of general critical nutrients glutamine and glucose 

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