Microbiological tests organic media

In addition to serologic testing, diagnosis should be pursued by microbiologic culture of blood or body fluid samples. Cultures should be held for at least 2 months, with weekly subcultures onto solid medium. Because it is extremely infectious for laboratory workers, the organism should be subcultured only in a biohazard hood. The reported frequency of isolation from blood varies widely, from less than 10% to 90% B melitensis is said to be more readily cultured than B abortus. Culture of bone marrow may increase the yield.46... 

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