Microanalysis carbohydrate

Daniel PF, De Feudis DF, Lott IT, McCluer RH (1981) Quantitative microanalysis of oligosaccharides by high performance liquid chromatography. Carbohydr Res 97 161-180 De Jong JG, Aerts JM, van Weely S, et al (1998) Oligosaccharide excretion in adult Gaucher disease. J Inherit Metab Dis 21 49-59... 

While the application of GLC to problems of microanalysis has had far reaching results in all areas of chemistry and biochemistry, the development of methods for use with carbohydrates and other polyhydroxy compounds was slower than with other classes of compounds. The major problem was the general lack of volatility of the polyhydroxy compounds and the fact that derivatives known to be volatile could not readily be prepared in quantitative yields by simple micro procedures. 

Breen, M. et al., Microanalysis and characterization of glycosaminoglycans from human tissue via zone electrophoresis, in Methods in Carbohydrate Chemistry, Whistler, R.L. and BeMiller, J.N., Eds., Academic Press, New York, 1976, p. 101. 

Microanalysis of Carbohydrate Mixtures by Isotopic, Enzymatic, and Colorimetric Methods... 

Breen M, Weinstein HG, Blacik LJ, Borcherding MS, and Sittig RA (1976) Microanalysis and characterisation of glycosaminoglycans from human tissue via zone electrophoresis. In Whistler RE (ed.) Methods of Carbohydrate Chemistry, vol. 7, pp. 101-115. New York Academic Press. 

Karlsson, K. A., 1974, Carbohydrate composition and sequence analysis of a derivative of brain disialoganglioside bt mass spectrometry, with molecular weight ions at m e 2245. Potential use in the specific microanalysis of cell surface components. Biochemistry 13 3643. 

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