Methylene blue electrochemical detection, nucleic acids

Label-free and label based electrochemical detection of hybridization by using methylene blue and peptide nucleic acid probes at chitosan modified carbon paste electrodes. Electroanalysis 14, 1685-1690. [Pg.154]

Enzyme DNA hybridization assays with electrochemical detection can offer enhanced sensitivity and reduced instrumentation costs in comparison with their optical counterparts. Efforts to prevent non-specific binding of the codissolved enzyme and to avoid fouling problems by selecting conditions suitable to amplify the electrode response have been reported by Heller and co-workers [107]. A disposable electrochemical sensor based on an ion-exchange film-coated screen-printed electrode was described by Limoges and co-workers for an enzyme nucleic acid hybridization assay using alkaline phosphatase [108] or horseradish peroxidase [109]. In another methodology to improve sensitivity, a carbon paste electrode with an immobilized nucleotide on the electrode surface and methylene blue as hybridization indicator was coupled, by Mascini and co-workers [110], with PGR amplification of DNA extracted from human blood for the electrochemical detection of virus. [Pg.401]

FefCN] ] " by methylene blue (MB] at a DNA-modified electrode. LB+ is leucomethylene blue, the product of the electrochemical reduction, (b] Cyclic voltammetry at a gold electrode modified with DNA of 2 mM [Fe(CN]6] (curve 1], 2 p.M MB (curve 2], and 2 mM [FefCNJe] and 2 p.M MB (curve 3]. Reproduced from S. 0. Kelly, E. M. Boon, J. K. Barton, N. M. Jackson, and M. G. Hill, Single-base mismatch detection based on charge transduction through DNA, Nucleic Acids Research, 1999, 27(24], 4830-4837, by permission of Oxford University Press. [Pg.213]

D. Ozkan, A. Erdem, P. Kara, K. Kerman, J. J. Gooding, P. E. Nielsen, and M. Ozsoz, Electrochemical detection of hybridization using peptide nucleic acids and methylene blue on self-assembled alkanethiol monolayer modified gold electrodes, Electrochem. Comm. 4[10], 796-802 [2002]. [Pg.401]