Methyl S -4-Cyano-3-hydroxybutanoate

7 One-pot Biocatalytic Synthesis of Methyl (5)-4-Chloro-3-hydroxybutanoate and Methyl (5)-4-Cyano-3-hydroxybutanoate 

Maja Majeric Elenkov, Lixia Tang, Bernhard Hauer and Dick B. Janssen 

1 Procedure 1 Expression and Purification of a Mutated Halohydrin Dehalogenase HheC-W249F 

1000 X stock solution of isopropyl / -D-l-thiogalactopyranoside (IPTG) (0.4 m) 1 g IPTG dissolved in 10 ml bidest water sterilize by filtering through a 0.22 pm filter 1000 X stock solution of ampicillin (50 mg ml ) 1 g ampicillin dissolved in 20 ml sterilized bidest water 

Luria-Bertani (LB) liquid medium and LB agar plates containing ampicillin 

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Procedure 2 Synthesis of Methyl (S)-4-chloro-3-hydroxybutanoate and Methyl (S)-4-cyano-3-hydroxybutanoate... 

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