Methyl Methanethiosulfonate MMTS

Methyl methanethiosulfonate (MMTS) is a small reversible blocking agent for sulfhydryl groups (Thermo Fisher, Toronto Research). It reacts with free thiols to form a dithiomethane modification with release of sulfinic acid (Figure 1.122). The sulfinic acid component decomposes into volatile products, which don t affect the disulfide formed from the MMTS reaction Alkylthiosulfonates react rapidly with thiols under mild conditions at physiological pH. The MMTS compound is a liquid at 10.6 M concentration and is conveniently added to a reaction medium by pipette. Complete thiol modifications of available cysteine residues in proteins can... 

Some volatile sulfur compounds were obtained from methionine and riboflavin after light irradiation under acidic conditions (Table I). Methyl mercaptan seems to be one of the important off-odor components. At this time, because of using the solvent extraction with dichloromethane, the amount of highly volatile methyl mercaptan seemed to be low. Therefore, we focused the other off-odor components, that is, methional, dimethyl disulfide (DMDS), dimethyl trisulfide (DMTS) and methyl methanethiosulfonate (MMTS). In particular, DMTS is considered to be significant because of its extremely low threshold value. Figure 1 shows that the amount of DMDS and DMTS increased with decreasing pH value. 

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See also in sourсe #XX -- [ ]

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