Methods to Obtain Bacterial Polymers

Various methods are involved in the isolation or extraction of bacterial polymers from various species. However, here we will only be providing information concerning a few common methods used for the isolation or extraction of various polymers. 

Polyhdroxyhutyrate Fifteen bacterial colonies of Pseudomonas pseudomallei were screened due to their ability to produce PHB [81]. These were isolated and preserved in suitable agar medium. Later, the culture medium was optimized for conditions with different carbon, nitrogen, and vitamin content in liquid synthetic medium for good yield of PHB. For large-scale production, the researcher prepared a 24h-old-culture in nutrient broth medium at 300°C. Later this culture medium was transferred to a 500 mL nutrient bath and incubated at 300°C while shaking (20 strokes/min) for 24h. At a later time, the cells were harvested by centrifugation at 8000 rpm for 12 min at 40°C. The harvested 

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