Methanobacterium strain MOH

Coenzyme M (CoM), 2-mercaptoethanesulfonic acid, was the first novel methanogen coenzyme described, and is the smallest organic coenzyme found in biological systems. The structure (Fig. 3) arose from work by Taylor, McBride, and Wolfe on methane production by extracts of Methanobacterium strain MOH, where a component was... 

The remainder of this paper discusses the nutritional requirements of three strains of methane bacteria grown with H2-CO2 as energy source —i.e., M. ruminantium strains isolated from sludge and the rumen and Methanobacterium strain MOH isolated from M. omelianskii 14). The latter is believed to be related closely to M. formicicum, except that it does not ferment formate (25). M. formicicum and M. ruminantium utilize... 

As with M. ruminantium, Methanobacterium strain MOH can utilize neither peptides nor amino acids as a nitrogen source replacing NH4 (Figures 5 and 7). NH4 utilization is essentially the same (Figure 8) when NH4 is the nitrogen source or when rumen fluid, amino acids, yeast extract, and Trypticase are present in the medium as complex sources of nitrogen that would be utilized in place of NH4 by most heterotrophic bacteria other than some rumen carbohydrate-fermenting anaerobes (11). 

Figure 7. Response of Methanobacterium strain MOH to growth-limiting concentrations of in the presence and absence of free i.-amino adds (19 mM a-amino acid nitrogen). The basal medium was that shown in Figure 5 but with 12.2 mM sodium acetate added. The proportions and kinds of amino adds were similar to those of casein. Numbers in parentheses indicate days of incubation for maximal OD.

Tzeng, S. F., Nutrition of Methanobacterium Strain MOH Isolated From... 

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