Methacrylated dextran prepared from

Columns of dextran have been prepared with a more elaborate procedure.13 It is claimed that the EOF has been eliminated and that columns are very stable under very harsh conditions, e.g., 0.05 M NaOH with 5% SDS or 1 M HC1. Cellulose-coated capillaries have been prepared by coupling 2-hydroxyethyl methacrylate (HEMA)-derivatized hydroxypropyl cellulose (HPC) to columns derivatized with 7-oct-l-enyltrimethoxysilane.The coating was stable for several weeks in the pH range from 2 to 10 and diminished the EOF, and it has been found useful for separation of peptides, glycoproteins, and derivatized oligosaccharides. 

In a variation of the above process, a water soluble monomer A is adsorbed on water-immiscible solvent droplets dispersed in the aqueous solution of A and then polymerized to form closed shell structure. For example, cross-linked polysaccharide capsules with diameters ranging from 200 nm to several microns and wall thicknesses of several tens of nanometers have been fabricated by interfacial polymerization of methacrylated N,N-diethylaminoethyl dextran (DdexMA) [212]. In a typical method of preparation of DdexMA (see Figure 5.85), a mixture of N,N-diethylaminoethyl dextran (Ddex),... 

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