Meth A tumor

We also tested the antitumor activity of CVS-U against rechallenge tumors using Meth A and CDF1 mice. We observed a strong antitumor effect against the rechallenge Meth A tumor after three i.t. administrations... 

We examined the antitumor effect of CVS against spontaneous tumor metastasis after surgically removing a s.c.-inoculated primary Meth A tumor mass from the right foot pad in BALB/c mice, Fig. (6)-Protocol A. The survival rates of the Meth A tumor-inoculated mice markedly increased when CVS was injected i.t. three times every other day following the tumor inoculation, Fig. (7)-A. A similar result was obtained in the EL-4 lymphoma and C57BL/6 syngeneic system (data not shown). 

CVS displayed a strong antitumor effect against experimental metastasis induced by i.v. tumor rechallenging, Fig. (6)-Protocol B. BALB/c mice were inoculated s.c. with Meth A tumor cells in the right flank on day 0 and rechallenged intravenously with the same tumor on day 9 or 58. The primary tumor was removed on day 11. A significant dose-dependent increase in survival rate was observed when CVS was injected i.t once on day... 

We evaluated delayed hypersensitivity to examine the participation of CVS administration on the cellular immunity to Meth A tumor antigens according to the delayed footpad reaction (DFR) [48] in the tumor i.v. rechallenge system. On day 9, 16, or 23, a quantity of 5 x 10 mitomycin C-treated Meth A cells in 0.05 ml PBS were injected into the right hind footpad of each BALB/c mouse. PBS injected into the left hind footpad... 

With these experiments it became clear that CVS is highly protective against adverse effects in 5FU-treated normal mice. We carried out similar experiments using the Meth A tumor-bearing mice. 

In normal mice, an intraperitoneal 250 mg/kg 5FU treatment is not lethal, but 100% mortality was observed within 2 weeks after the same dose of 5FU treatment in mice bearing Meth A tumors. The mean survival time of Meth A-inoculated, 5FU-treated mice (23.1 1.2 days) is significantly shorter than that of 5FU-untreated tumor-bearing mice (29.0 3.5 days). In CVS-administered tumor-bearing mice, the mean survival time is 48.5 8.7 or 37.6 6.2 days with or without 5FU treatment, respectively. With respect to the antitumor effect as determined by tumor growth, 5FU was... 

Fig. (13). Effect of a combination of CVS and 5FU on body weight (A) and tumor growth (B) in tumor bearing mice. All mice were inoculated s.c. with 5x10° Meth A tumor cells on day 0. CVS was injected s.c. near the tumor on day 1,3,6,8,11 and 13. 5FU was treated i.p. at a dose of 250 mg/kg on day 14. Solid line means 5FU-nontreated, and dotted line means 5FU-treated mice ( ) means CVS-noninjected and (o) means CVS-injected mice.

Stucker, O., Vicaut, E., and Teisseire, B. (1992) Specific response to 5-HT2 agonists by arterioles linked to Meth A tumors in mice. Am. J. Physiol. 262 (Pt 2), H704—H709. 

The growth of transplantable Meth A tumor was significantly suppressed in an Ag-specific manner when CVE was administered by not only an i.p. route [30], but orally as well [31]. It has been demonstrated that glycoprotein-rich substance (CVS) from the culture supernatant of Chlorella vulgaris strain CK displayed antitumor effect against experimental metastasis induced by Meth A fibrosarcoma [32]. 

Sun, S. et al.. Anti-tumor activity of astaxanthin on Meth-A tumor cells and its mode of action, FASEBJ., 12,A966,1998. 

K) with different degrees of carboxymethylation after a single iv dose to Meth A tumor-... 

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