Metabolomics and metabolism

Genomics, metabolomics and metabolic flux analysis, thermodynamic feasibility, computer demonstration... 

Kraly, J. R., Holcomb, R. E., Guan, Q., and Henry, C. S. 2009. Review Mieroflnidie applieations in metabolomics and metabolic profiling. Anal. Chim. Acta 653 23-35. 

Chen, C., Krausz, K. Idle, J. and Gonzalez, F.J. (2008) Identification of novel toxicity-assodated metabolites by metabolomics and mass isotopomer analysis of acetaminophen metabolism in wild-type and Cyp2el-null mice. Journal of Biological Chemistry, 238 (8), 4543 559. 

Kristensen C, Morant M, Ollsen CE, Ekstrom CT, Galbraith DW, MoUer BL, Bak S. (2005). Metabolic engineering of dhurrin in tiansgenetic Arabidopsis plants with marginal inadvertent effects on metabolome and transcrip tome. Proceedings of the National Academy of Sciences, 102,... 

While the clinical use of MS/MS of hormonal steroids is new, metabolite analysis by gas chromatography (GC)-mass spectrometry (MS) has been available for 40 years, since few immunoassays were developed for urinary analytes. Profile analysis is a very powerful technique and it must be recognized that with few exceptions, all disorders of steroid synthesis and metabolism first had their metabolome defined... 

Section 1.4). If we knew the composition of a cell s metabolome, we could predict which enzymes and metabolic pathways were active in that cell. 

The LTQ-FT mass spectrometer was introduced in late 2003 and, as expected, the main application discussed in the literature is for the analysis of proteins and peptides (Johnson et al., 2004 Syka et al., 2004). A recent book chapter (van der Greef et al., 2004) and a review article (Brown et al., 2005) discussed the application of the LTQ-FT to metabolomics. FTMS applications to dmg metabolism are still very new and dmg discovery research laboratories which have recently purchased the instmment are still in the process of developing and validating methods and approaches. A recent publication describes the depth and flexibility of the experimental setup utilizing accurate mass data-dependent exclusion MS" measurements with a LTQ-FT (Tozuka et al., 2005). We have reported several integrated approaches for determination of metabolic stability, characterization of metabolites and metabolic... 

One of the earliest and perhaps most rewarding introductions of metabolomics in pediatric medicine has occurred with the bioanalysis of endogenous substrates and altered metabolism that may be indicative or predictive of significant disease states. Newborn and metabolic screening of inherited disorders of metabolism has, in many respects, served as a cornerstone for innovative... 

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