Merrifield solid phase method

Automated Peptide Synthesis The Merrifield Solid-Phase Method 1037... 

Peptide synthesis requires the use of selective protecting groups. An N-protected amino acid with a free carboxyl group is coupled to an O-protected amino acid with a free amino group in the presence of dicydohexvlcarbodi-imide (DCC). Amide formation occurs, the protecting groups are removed, and the sequence is repeated. Amines are usually protected as their teit-butoxy-carbonyl (Boc) derivatives, and acids are protected as esters. This synthetic sequence is often carried out by the Merrifield solid-phase method, in which the peptide is esterified to an insoluble polymeric support. 

Show a synthesis of Ala-Phe-Gly-Gly using the Merrifield solid phase method. 

Solid-phase peptide synthesis. In the Merrifield solid-phase method for peptide synthesis (1, 516-518), vigorous conditions are usually necessary for... 

This pentapeptide (asp-ser-asp-pro-arg) was prepared by the Merrifield solid phase method [690]. Pentigetide has the same amino acid sequence as... 

Merrifield Solid Phase Methods for Chemical Synthesis of Repeating Sequences... 

Although the steps just shown can be repeated to add one amino acid at a time to a growing chain, the synthesis of a large peptide by this sequential addition is long and arduous. An immense simplification is possible, however, using the Merrifield solid-phase method. In the Merrifield method, peptide synthesis is carried out with the growing amino acid chain covalently bonded to small beads of a polymer resin rather than in solution. In the original Merrifield procedure, polystyrene resin was used, prepared so that 1 of... 

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