NCTC media

The Connaught Medical Research Laboratories Media are also designed to support cell growth without the addition of serum. Thus CMRL 1066 (Appendix 1 Table 20) will support the growth of several cell types as will the National Cancer Institute medium NCTC 135 (Appendix 1 Table 19) and CMRL 1415 comes nearest to supporting the growth of cell strains (Healy and Parker, 1966a,b Evans et al., 1964). See 5.8 for more details. 

All normal hearts used demonstrated ventricular contraction prior to introduction into the pump. As soon as perfusion started with the NCTC 135-new born calf serum medium, it immediately induced strong rapid ventricular contractions (130 to 160 beats per minute). 

Waymouth, C., 1959, Rapid proliferation of sublines of NCTC clone 929 (Strain L) mouse cells in a simple chemically defined medium (MB 752/1), J. Natl. Cancer Inst. 22 1003-1017. 

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