Measuring Conformational Dynamics of Proteins by Hydrogen Exchange

1 Measuring Conformational Dynamics of Proteins by Hydrogen Exchange 

HX-MS at both whole protein and peptide resolutions offers important insights into a protein s dynamic properties in solution through its physicochemical properties. At peptide resolution, HX-MS describes the relative exchange across different regions of the protein, contingent on the acid-stable protease s fragmentation profile (see Section 6.2.2). If high-resolution structures of the protein are available, the output of an HX-MS experiment can be overlaid on the structural model of the protein and represented by a heat map of the protein of interest. 

Hydrogen Exchange Mass Spectrometry of Proteins Fundamentals, Methods, and Applications, First Edition. Edited by David D. Weis. 

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