Measurement of intracellular pH

C.K. Rhee, L.A. Levy, R.E. London, Fluorinated o-aminophenol derivatives for measurement of intracellular pH, Bioconjug. Chem. 6 (1995) 77-81. 

One of the simplest applications of MRS is in the measurement of intracellular pH. The chemical shifts characteristic of H2PO4 and HPO differ by only 2.4 ppm and the equilibrium between the two types of anion in the body results in only one observed resonance peak. The exact position of this resonance peak depends on the ratio of the two anionic species, and thus it can be used to estimate pH. 

Halm M, Hornbaek T, Arneborg N, Sefa-Dedeh S, Jespersen L. (2004). Lactic acid tolerance determined by measurement of intracellular pH of single cells of Candida krusei and Saccharomyces cerevisiae isolated from fermented maize dough. Int J Food Microbiol, 94, 97-103. 

Brook, P. F. Lawry, J. Cooke, 1. D. Barratt, C. L. R. Measurement of intracellular pH in human spermatozoa hy flow cytometry with the benzo[c]xanthene dye SNAFL-1 A novel, single excitation, dual emission, molecular probe. Mol. Hum. Reprod. 1996, 2, 18-25. 

Carter, N.W., Rector, F.C., Campion, D.S., Measurements of Intracellular pH of Skeletal Muscle with pH Sensitive Glass Microelectrodes, J, Clin. Invest., 920-933 (1967). 

The measurement of intracellular pH from the chemical shift of inoranic phosphate (Pi) with reference to three independent peaks has been investigated by Rata et al. in ten volunteers and eight patients with non-Hodgkin s lymphoma. Measurements using the PCr peak, where... 

The measurement of intracellular pH by NMR is an important application of this technique. For both cell suspensions and perfused organs the techniques that are used most often today involve the measurement of the equilibrium distribution of acid or base indicators across cell membranes. Questions arise concerning possible binding of the indicator to intracellular compounds and membranes, thereby introducing contributions other than pH to the distribution. In the case of ischemic organs, difficulties arise because these methods require flow (Poole-Wilson, 1978). Thus,... 

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