Mean residue weight

Protein concentrations were approximately 0.4%. Circular dichroism data were presented as mean residue ellipticity [0], in degrees X cm x decimole . The same mean residue weight 112.4 was employed for native lysozyme and its ozonized products, since the deviation caused by the ozonolysis of few amino acid residues of lysozyme is far less than the experimental error. 

Another term that is frequently used is [0]mrw> where MRW stands for mean residue weight. 

Fig. 11. (a) Far ultraviolet rotatory dispersion of ribonuclease. Corrected mean residue specific rotation vs. wavelength [to R = [aLAf/100 [3/(n2 + 2)l where a — specific rotation, M mean residue weight, and n = solvent refractive index. Bars give maximal deviation at peaks. Reproduced from Jirgensons (311). (b) Near ultraviolet rotatory dispersion of 0.48% pancreatic ribonuclease in a 1-mm cell, in (a) 0.15 M phosphate buffer at pH 62 (b) 0.15 M glycine-NaOH buffer at pH 11.5 (c) 0.1 N HC1 (d) 15% sodium dodecyl sulfate. Reproduced from Glazer and Simmons (313). (c) Far ultraviolet circular dichroic spectra of RNase-A, RNase-S, and S-protein at 25° and 3°. Reproduced from Pflumm and Beychok (313). (d) Near ultraviolet circular dichroic spectra of RNase-A as a function of pH. Reproduced from Pflumm and Beychok (313). 

Fig. 5 Poly(L-glutamic acid) containing 56 mol% azobenzene units in the side chains (III). CD spectra in trimethylphosphate before (continuous line) and after (dashed line) irradiation at 360 nm. Below 250 nm, molar ellipticity is based on the mean residue weight above 250 nm, the ellipticity is referred to one azo-Glu residue.

Figure 4. Circular dichroism spectra from 200 to 250 nm for ovotransferrin. Mean residue weight of 112 is used.

Residues of amino acid per 1000 total residues. Assuming mean residue weight is 91. 

Fig. 10 (74). OKD (—), MORD (—) and corrected MORD (.) spectra of bovine erythrocuprein. MORD was corrected by substracting the ORD portion. Molar rotations were calculated from 220—290 nm on the basis of mean residue weight, and from 300—600 nm on the basis of the molecular weight of erythrocuprein. The values are not corrected with respect to the refraction index of the solvent...

Commonly, for the sake of comparison, the molar ellipticity [6>] = 100 Ofcd in units of deg cm dmol" is recorded, where c is the concentration in mol and d is the optical path length. If, in the case of polymers such as proteins, the molar concentration is related to the molar mass of the residue, i.e. to the repeating (base) unit, the mean residue weight ellipticity [0]mrw is obtained. 

The total mass of the Escherichia coli ribosome is 2,520,000 Da. Considering only the protein that constitutes only one third of the ribosome, the molecular weight of the protein is 857,000 Da. Assuming the mean residue weight to be 100, there are some 8,570 residues. The formation of 8,570 peptide bonds alone would cost 17,000 ATP equivalents. Thus, the assembly of only the tRNA and protein components required for translation at the ribosome number requires much more than 23,000 ATP equivalents. Of course, a ribosome can be used for the synthesis of many copies of protein, but, even with synthesis of a large number of proteins before the ribosome would have to be replaced, the number of ATP equivalents is significant. 

CD of CA with and without LPS was measured with a JASCO Model J 500A spectropolarimeter. Amounts of a-helical conformational modes in CA were determined from ellipticity values at 222°nm (Chen and Yang, 1971), adjusted for the presence of LPS. The mean residue weight of CA was 108.2 g mole ... 

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