Circular dichroism data

Protein concentrations were approximately 0.4%. Circular dichroism data were presented as mean residue ellipticity [0], in degrees X cm x decimole . The same mean residue weight 112.4 was employed for native lysozyme and its ozonized products, since the deviation caused by the ozonolysis of few amino acid residues of lysozyme is far less than the experimental error. 

In this section, we first discuss predictions of ab initio computations in comparison to photoemission and x-ray magnetic-circular dichroism data, discussed already in the previous sections. Theoretical models employing parameterized Hamiltonians are presented next emphasizing their successes and shortcomings in the description of magnetic phenomena. 

There is no generalized treatment of the UV/visible spectra of these complexes since they tend to be dominated by low-energy charge transfer bands which obscure details of the d-d structure. In a few instances, circular dichroism data have been reported, but detailed interpretation has not been attempted (19). 

Greenfield, N.J. 1996. Methods to estimate the conformation of proteins and polypeptides from circular dichroism data. Anal. Biochem. 235 1-10. 

Based on combined EPR and magnetic circular dichroism data obtained for the BFRs of P. aeruginosa, A. vinelandii, and E. coli, bis-... 

Circular dichroism data for some, -(d)- and A-(i)-tris(chelate) complexes of cobalt(lll)°... 

Because of the approximation that = Aobsc this approach will give rise to minimal correction values and an iterative procedure can be used to obtain the desired accuracy [112]. If the particulate system is best described as a vesicle, which is generally the case for membranes, an equivalent A for Qa (vesicles) [114] can also be obtained from Table 3. Of course, the problem at this stage is that in general an equivalent solution absorption spectrum will not be known for the suspension of interest. By also utilizing the circular dichroism data, however, an equivalent solution absorption spectrum can be determined as outlined below. This is called the pseudoreference state approach. 

The configuration of homoproaporphines at the spiro asymmetric centre may be deduced from circular dichroism data for the bases and their derivatives. ... 

Circular dichroism data for a series of a-ketols and their acetates show that axial acetoxy-groups frequently make anti-Octant contributions to the ketone (n 7T ) Cotton effect. Axial hydroxy-groups may produce either an Octant or anti-Octant effect. It seems probable that the conformation of the acetoxy-or hydroxy-group itself may be the decisive factor, the orientations of oxygen lone-pairs in relation to the carbonyl group probably being critical. 

Estimated on the basis of the circular dichroism data of Ananthanarayanan and Hew (35). 

An interesting variation of the catalysis by poly(amino acids) was reported by Hatano et al. (141). Several metal ions and their complexes are known to be effective catalysts for the hydrolysis of amino acid esters, in wdiich coordination of substrates to the metal ion renders nucleophilic attack by H2O more facile 142). Hatano et al. found ttat D-phenylalanine methyl ester 20 was catalytically hydrolyzed by poly-L-lysine-Cu(II) complex 3 to 4 times more efficiently than the L-iso-mer. The rate difference arose from the lower enthalpy of activation for the D-sub-strate. At die same time, the circular dichroism data indicated that formation of the catalj t-substrate complex was more favorable with the D-ester than with the L-ester (74J). Ap endy, poly-L-lysine helped form an asymmetrically selective binding site. 

Where CD (circular dichroism) data exist, the solvent used is in parentheses. The values are in As (positive or negative) and the corresponding A nax (nm) is in parentheses. 

Secondly, in the light of dialysis experiments (and related spectral data) the dye binding process is likely to depend notably on the presence of the aromatic ring. This may involve this g oup directly by some ability to interact preferentially with dye molecules (as also implied from induced circular dichroism data) and/or indirectly as inducing micelle-like domains along the macromolecular chains. But it has to be... 

Table 1 Comparisons of methods of analysing protein conformation from circular dichroism data ...

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