Maximum reaction velocity, glucose

The reaction rates, 91, 91 and 913, are in terms of the mole concentration of glucose, maltose and maltotriose, 5, S2 and. The maximum reaction velocities, Michaelis constants and inhibition constants in the reaction rates follow the Arrhenius dependency. The definitions of the symbols and their corresponding data can be obtained from the literature (Gee and Ramirez, 1988 Wang and Jing, 2(XX)). 

For blood glucose monitoring, it will generally be necessary to obtain linear sensor response in the range of 2-20 mM. In an air-saturated solution, the Michaelis constant (Km) for glucose will be 5-6 mM. This corresponds to the substrate concentration yielding half the maximum reaction rate velocity. The maximum rate would occur at a concentration of about 15 mM, leading to mostly nonlinear... 

For GOD, apparent Michaelis-Menten constants K, the substrate concentration at which the reaction velocity is half the maximum velocity, Vmaxl l) ranging from 4 mM to 13 mM have been cited for different immobilization conditions [36]. In excess glucose (i.e, glucose concentrations sufficiently greater ), the rate of hydrogen peroxide generation can be approximated by... 

Immunoassay standard curves. Immunosensors were prepared as described. Sample, standards, and conjugate were added sequentially to the respective cells to start the competitive assay. After a 10 min incubation, the solution was discarded and a proprietary buffer was added to each cell. A sufficient concentration of glucose was then added to each cell to drive the enzyme reaction to maximum velocity. As in the qualification tests, the hydrogen... 

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