Matrix-assisted laser oligosaccharide structures

Fig. 4.5.8 Examples for structures and molecular masses of 2-aminobenzamide (2-AB)-labelled oligosaccharide moieties derived from serum transferrin. Values below the oligosaccharide structures indicate the expected masses (in Da) by matrix-assisted laser desorption ionisation - time of flight analysis.

C. S. Creaser, J. C. Reynolds, and D. J. Harvey, Structural analysis of oligosaccharides by atmospheric pressure matrix-assisted laser desorption/ionisation quadrupole ion trap mass spectrometry, Rapid Commun. Mass Spectrom., 16 (2002) 176-194. 

B. Spengler, D. Kirsch, R. Kaufmann, and J. Lemoine, Structure analysis of branched oligosaccharides using post-source decay in matrix-assisted laser desorption ionization mass spectrometry, Org. Mass Spectrom., 29 (1994) 782-787. 

Lemoine, J., Chirat, F. and Domon, B. (1996) Structural analysis of derivatized oligosaccharides using post-source decay matrix-assisted laser desorption ionization mass spectrometry. J. Mass Spectrom., 31 (8), 908-12. 

MALDl-TOF MS stands for Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry. It is a highly sophisticated technique (also used for proteins, see text Chapter 4, Section 4.1.7), but it can t solve oligosaccharide structures without input from other techniques. Why not ... 

T. J. P. Naven and D. J. Harvey, Effect of structure on the signal strength of oligosaccharides in matrix-assisted laser desorption/ionization mass spectrometry on time-of-flight and magnetic sector instruments. Rapid. Commun. Mass Spectrom., 10 (1996) 1361-1366. 

In the early nineties nuclear magnetic resonance (NMR) spectroscopy was the preferred technique in order to fully characterise structures of oligosaccharides. Nowadays, both matrix assisted laser desorption/ ionisation time-of-flight (MALDI-TOF) and electrospray MS have become routine and powerful techniques in the identification of structural features of oligosaccharides (26-28), even to determine the positions of residues within oligosaccharides (29,30). 

Matrix-assisted laser desorption—ionization mass spectroscopy is a convenient method for rapid and sensitive structural analysis of oligosaccharides (Harvey 1999). It was observed that MALDI TOE TOF mass spectrometric analysis reveal a series of masses (m/z) corresponding to pentose and hexose sugars... 

Morelle, W. Slomianny, M.-C. Diemer, H. Schaeffer, C. van Dorsselaer, A. Michalski, J.-C. Structural characterization of 2-aminobenzamide-derivatized oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight tandem mass spectrometer. Rapid Commun. Mass Spectrom. 2005, 19, 2075-2084. 

Suzuki, M. Suzuki, A. Structural characterization of fucose-containing oligosaccharides by high-performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Biol. Chem. 2001, 382, 251—257. 

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