MAP, Mitogen-activated protein

The OP group of receptois share common effector mechanisms. All receptois couple via pertussis toxin-sensitive Go and Gi proteins leading to (i) inhibition of adenylate cyclase (ii) reduction of Ca2+ currents via diverse Ca2+ channels (hi) activation of inward rectifying K+ channels. In addition, the majority of these receptors cause the activation of phospholipase A2 (PLA2), phospholipase C 3 (PLC 3), phospholipase D2 and of MAP (mitogen-activated protein) kinase (Table 3). 

Schematic diagram of the insulin receptor heterodimer in the activated state. IRS, insulin receptor substrate MAP, mitogen-activated protein P, phosphate tyr, tyrosine.

Fig. 2 Presynaptic signaling mechanisms of ai adrenoceptor subtypes (Oia, 2B, Ofec)- Abbreviations W2abc> Pi23> adrenoceptor subtypes MAP, mitogen-activated protein kinase.

MALDI-TOF Matrix-assisted laser desorption ionization time-of-flight MAP Mitogen-activated protein, nsnally referring to a protein kinase such as M AP-... 

MAP mitogen-activated protein NDMA N-methyl-D-aspartate... 

PTX, pertussis toxin PLC, phospholipase C PTP, phosphotyrosine phosphatase AC, adenyl cyclase MAP, mitogen-activated protein (kinase). 

In order to determine the selectivity of each peptide sensor substrate, assays with a panel of recombinantly expressed, activated kinases can be performed (Figure 1.7b). In this case, the concentration of substrate peptide is held constant at two to three times the determined K. For example, the p38 sensor depicted in Figure 1.6 was incubated with various MAP (mitogen-activated protein) and non-MAP kinases and fluorescence emission was monitored. Figure 1.7b demonstrates the selectivity of the p38 sensor for the target kinase [23]. Following this initial screen, kinase selectivity can be addressed directly in cell lysates. Cells can be stimulated to activate the kinase of interest and kinase activity can be determined in the presence and absence of a selective inhibitor of the kinase of interest. Residual activity in the presence of inhibitor would indicate sensor cross talk with other kinases. 

Bird TA, Schule HD, Delaney PB et al (1992) Evidence that MAP (mitogen-activated protein) kinase activation may be a necessary but not sufficient signal for a restricted subset of responses in IL-l-treated epidermoid cells. Cytokine 4 429-440... 

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