Man-BSA

The neoglycoproteins have also been used to discriminate between clearance systems having similar specificities. By use of the amidin-ated neoglycoproteins, it has been shown by inhibition experiments that Man-BSA and GlcNAc-BSA are cleared, at least in part, by the same system in the intact rat.150 This system also clears Glc-BSA. In the nephrectomized, eviscerated rat, however, the a-D-mannopyrano-... 

Diffusion in agar performed by the conventional method shows a strong reaction between the antibody and Man-BSA, but not with BSA (Fig. 12, plates A). However, both compounds give precipitin complex with anti-BSA serum. Oxidation of the antigens with periodate no longer gives a precipitin reaction with the anti-mannose antibodies, but has no effect on the anti-BSA antibodies. Hapten-inhibition results are also shown in Fig. 12, plates C and D. These tests are conducted with the purified antibody... 

Fig. 12.—Reactivity of anti-a-mannose antibodies and anti-BSA antibodies with the native and periodate-oxidized mannose-glycoconjugate and BSA (plates A and B). M = Man-BSA, xM = periodate-oxidized Man-BSA, B = BSA, xB = periodate-oxidized BSA, A = antimannose antibodies, A2 = anti-BSA antibodies. Plates C and D show hapten inhibition of purified anti-mannose antibodies (A ) with mannose (L). The amount of antigen in the outer wells ranges from 20 /u.g in well 1 to 1 /u.g in well 6. [Reprinted with permission from J. H. Pazur, B. Liu, and T. Witham. J. Protein Chem., 13 (1994) 59-66.]...

HAS, human serum albumin BSA, bovine serum albumin Sue, succinic acid Aco, aconitic acid Form, formaldehyde Man, mannose Mai, maltose Nap, naproxen Dexa, dexamethasone. ND, not done -, no uptake +, small uptake ++, moderate uptake +++, abundant uptake. 

Fig. 13.—A Agar diffusion of immune sera (Se), 1-thio-D-mannose antibodies (A ), and anti-BSA antibodies (A2) against Man-S-BSA (/) and BSA (2). B Agar-diffusion plate of anti-Man-S-antibodies and antibodies oxidized by peroxypropanoic acid for 0, 4, and 8 h. C, D, E, and F Hapten inhibition by agar diffusion, A = purified anti-Man-S antibodies I = antibodies + p-nitrophenyl 1 -thio-a-D-mannopyranoside 12 = antibodies + D-mannose I3 = antibodies + ethyl 1-thio-a-o-mannoside 1 to 6, outer wells contain decreasing concentration of Man-S-BSA. (Reprinted with permission from Journal of Protein Chemistry, Volume 9, J. H. Pazur, B. Liu, Nan Q Li, and Y. C. Lee, pp. 143-150, copyright 1990 Journal of Protein Chemistry.)...

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