Malachite green reagent

MACB = 5-chloro-2-(methylamino)benzo-phenone 227 Macrolide antibiotics 195 Magnesium cations 144,145, 311, 312 Malachite green reagent 45 Malathion 362... 

Malachite Green Reagent Dissolve 50 mg malachite green (final concentration 1 mg/ml) (Ajax Chemicals, NSW, Australia) and 500 mg ammonium molybdate tetrahydrate (final concentration 100 mg/ml) (Ajax Chemicals) in 50 ml of 1 M hydrochloric acid (HCl) followed by filtration through a 0.45 gLm filter. Store the solution at room temperature (22°) in the dark for a maximum of 8 weeks. 

In the malachite green procedure, 10 ml of the sample solution containing up to 0.7 xg phosphorus as orthophosphate was transferred into a 25 ml test tube. To this solution was added 1 ml each of 4.5 M sulfuric acid and the reagent solution. The solution was shaken with 5 ml of a 1 3 v/v mixture of toluene and 4-methylpentan-2-one for 5 min. After phase separation, the absorbance of the organic phase was measured at 630 nm against a reagent blank in 1 cm cells. 

Carboxylic acid Malachite green, Cystamine-Ellman s reagent - ... 

Colored reagents to follow the appearance or the disappearance of a functional group have been widely used to monitor reactions in classical organic chemistry, particularly in TLC analysis. This technique has been successfully adapted to SPS for example, ninhydrin (118), bromophenol blue (119), nitrophenyl isothiocyanate-O-trityl (120), picric acid (121), and malachite green isothiocyanate (122) have all been used to show the presence or the absence of free resin-bound amines. The presence of free resin-bound thiol groups can also be detected (123). 

Catalysts have been used to make end points detectable these have been reviewed by Mottola. In such systems the titrant reacts rapidly with the substance titrated, but excess reagent reacts only slowly with an indicator in the absence of an appropriate catalyst. For example, small amounts of complexing agents such as EDTA can be determined by titration with Mn(II). With malachite green as indicator in the presence of periodate, the excess Mn(II) catalyzes the indicator oxidation. 

In its general reactions it is like all aldehydes. It is an important reagent in all cases where an aldehyde is needed and in the manufacture of certain dyes, e.g., malachite green (p. 747). 

The anionic complex of Sb(III) with mandelic acid forms with Malachite Green an associate which is extractable into chlorobenzene from weakly acid solutions (e = 6.T10 ) [54]. Mandelic acid and other organic reagents have been applied for the joint determination of Sb(ffl) and Sb(V) [55,56]. 

Methods for chromium with use of triphenylmethane reagents are less sensitive examples are Chrome Azurol S (e = 5.9-10 ) [43,44], Xylenol Orange (e = 1.0-10" ) [45], and Malachite Green [46]. A much higher sensitivity is obtained when Cr(III) is determined with the use of Eriochrome Cyanine R in the presence of surfactant CTA (e = 6.8-10" ) [47]. Cr(lll) was determined with Eriochrome Cyanine by means of the derivative spectrophotometry [48]. 

The following spectrophotometric reagents were applied for determining indium 2-(2-thiazolylazo)-p-cresol (TAC), in catalysts [47] Malachite Green, in gallium metal and in ZnGeAs2 [53] and Pyrogallol Red, in zinc alloys [7]. 

Combi cassette. The ColorLock Gold assay kit is based on malachite green-molybdate colorimetric assay, and the yellow reagent turns green in the presence of phosphate. The stabilizer stabilizes acid-labile substrates and the molybdate complex to maintain signal stability. 

The Pj liberated by acid or enzymatic hydrolysis of the inositol phosphates may also be assayed. An anion-exchange HPLC column, which separates the inositol phosphates, is coupled to a second column that contains immobilized alkaline phosphatase. The Pj that is released by the enzyme is measured color-imetrically. The system can detect 1 nmol of inositol phosphates in a single sample to indicate levels of IP3 of 13 to 40 nmol/g of tissue. The sensitivity of the phosphate assay is increased to the picomolar level with malachite green as a reagent (Dean and Beaven, 1989 Palmer and Wakelam, 1989). 

This test is based on bromide s redox characteristic and the ability of bromine to give a color reaction with a solution of decolorized fuchsin. This reagent is also known as malachite green and by many authors as Schiff s reagent, owing to its use in a determination of aldehydes discovered by H. Schiff. 

It has been reported that y3-CD could improve the selectivity of the color reactions of various metal ions with triphenylmethane, xanthene acid dyes and some other coloring reagents. The effect of fi-CD on the association compound system of metal (Mo, Zn, Co)-thiocyanate basic dyes such as malachite green, crystal violet, rhodamine B, rhodamine 6G and butyhhodamine B, has been investigated and the result shows that /3-CD could contribute to a more sensitive and stable system which improve the solubility of the basic dyes and produce a favorable microenviromnent for the color reactions [63]. /3-CD could be employed to solubilize the 1,2-amino anthraquinone in water due to the formation of inclusion complex which acts as a ligand for metal ions could be used for the determination of palladium at trace levels by spectrophotometry. In the spectrophotometric determination of microamounts of Zn based on the Zn-dithizone color reaction, -CD could increase the apparent molar absorptivity at 538 nm by 8.37 times. In the presence of cr-CD, the determination sensitivity of copper in leaves based on the color reaction of Cu(II) and mesotetrakis (4-methoxy-3-sulfophenyl) porphyrin was enhanced by 50% in the spectrophotometric analysis [64,65]. 

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