Major differences between various dyes

Commercial dyeing Eco-friendly dyeing Vegetable dyeing Organic dyeing Herbal dyeing 

Since when Developed 80 years ago Developed 10-15 years ago Developed 60 years ago Developed 500 years ago. Time tested 

Pollution Leads to heavy pollution. Uses 8000 chemicals Pollution under check, but still uses 8000 chemicals Cheap synthetic chemicals and dyes are still used No pollution whatsoever as colors are from herbs, flowers, stems and roots 

Allergies Mostly allergic, pro-microbial inflammatory and bad for respiration Could be allergic, pro-microbial inflammatory and bad for respiration Depends on chemicals used The herbs used cure allergies and have antimicrobial, antiinflammatory properties 

Cancer 42 cancer- causing chemicals could be present Only 22 cancer-causing amines are eliminated Depends on chemicals used No carcinogenic amines present 

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The most common methods used to determine protein concentration are the dye-binding procedure using Coomassie brilliant blue, and the bicinchonic-acid-based procedure. Various dyes are known to bind quantitatively to proteins, resulting in an alteration of the characteristic absorption spectrum of the dye. Coomassie brilliant blue G-250, for example, becomes protonated when dissolved in phosphoric acid, and has an absorbance maximum at 450 nm. Binding of the dye to a protein (via ionic interactions) results in a shift in the dye s absorbance spectrum, with a new major peak (at 595 nm) being observed. Quantification of proteins in this case can thus be undertaken by measuring absorbance at 595 nm. The method is sensitive, easy and rapid to undertake. Also, it exhibits little quantitative variation between different proteins. 

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