Magic-angle spinning membrane proteins

Abstract Membrane proteins represent an important and challenging frontier in structural biology they mediate fundamental and medically important processes but are challenging targets for structure/ function studies. Magic angle spinning solid-state NMR methods for structure determination of proteins have developed rapidly in... 

Solid-state NMR also can be applied to membrane proteins in lipid bilayers, and recent advancements in magic angle spinning solid-state NMR show promise for stmcture determination. Although the stmctures of small crystalline proteins (89) and membrane bound peptides (90) have been determined, the stmcture of a polytopic membrane protein has yet to be reported. The major necessity that is required to push the technique forward is the de novo sequential chemical shift assignment of the amino acid residues, and in the last few years, several groups have reported successful strategies (91, 92). 

McDermott A (2009) Structure and dynamics of membrane proteins by magic angle spinning solid-state NMR. Ann Rev Biophys 38 385 03... 

Li Y, Berthold DA, Gennis RB et al (2008) Chemical shift assignment of the transmembrane helices of DsbB, a 20-kDa integral membrane enzyme, by 3D magic-angle spinning NMR spectroscopy. Protein Sci 17 199-204... 

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