Mag-fluo

FIG. 1. A confocal image from a freshly isolated uterine myocyte from pregnant rat. The SR was loaded with mag-fluo-4 to show its structure and distribution. The stack of images was acquired using a Perkin Elmer Ultra VIEW LCI confocal imaging system. Images were combined to visualize the 3D distribution of the SR (A.V. Shmigol S. Wray, unpublished data). 

FIG. 5. Simultaneous and reproducible measurements of changes in SR and cytosolic Ca2+ in response to agonists. Pregnant rat myometrial cells were superfused with Krebs (2 mM Ca2+) and Carbachol (in Ca-free solution) applied as indicated. Repetitive applications of Carbachol evoked reproducible [Ca2+]j elevations (bottom trace) and SR [Ca2+]L decreases (upper traces). Left-hand axis cytosolic Ca2+. Right-hand axis intraluminal (SR) Ca2+changes, measured with mag-fluo-4. (Taken from Shmigol et al 2001.)... 

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