Macrophage oxidative stress

CN133 Oliveros, L. B., A. M. Videla, and M. S. Gimenez. Effect of dietary fat saturation on lipid metabolism, arachi-donic acid turnover, and peritoneal macrophage oxidative stress in mice. Braz J Med Biol Res 2004 37(3) 311-320. 

Figure 8.5 Pomegranate by-product (PBP) consumption by E° mice attenuates atherosclerotic lesion development, in association with reduction in macrophage oxidative stress and Ox-LDL uptake. E° mice consumed PBP (17 or 51.5 mg gallic acid equivalents/kilogram/day) for 3 months. Control mice received only water (placebo). At the end of the study, the mice aortas as well as the mice peritoneal macrophages were harvested. (A) Atherosclerotic lesion size determination. (B) Total macrophage peroxide levels were determined by the DCFH-DH assay. (C) For determination of macrophage paraoxonase 2 (PON2) lactonase activity, cells (2 x 10e) were incubated with 1 mmol/L dihydrocoumarin in Tris buffer, and the hydrolysis rate was determined after 10 min of incubation at 25°C. (D) The extent of Ox-LDL (25 pg of protein/ milliliter, labeled with FITC) uptake by the mice macrophages (1 x 10e) was determined by flow cytometry. Results are expressed as mean S.D. of three different determinations. = p < 0.01 versus placebo.

Rosenblat M., Volkova N., Coleman R., and Aviram M., Pomegranate by-product administration to apolipoprotein E-deficient mice attenuates atherosclerosis development as a result of decreased macrophage oxidative stress and reduced cellular uptake of oxidized low-density lipoprotein, J. Agric. Food. Chem., 54, 1928, 2006. 

Keidar S, Hayek T, Kaplan M, et al. Effect of eplerenone, a selective aldosterone blocker, on blood pressure, serum and macrophage oxidative stress, and atherosclerosis in apolipoprotein E-deficient mice. J Cardiovasc Pharmacol 2003 41 955-963. 

The progression of human immunodeficiency virus (HIV) towards its more advanced stages is accompanied by increasing body stores of iron. Iron accumulates in macrophages as well as microglia, endothelial cells and myocytes. The iron burden is especially intense in the bone marrow, brain white matter, muscle and liver. Such excesses of iron will enhance oxidative stress, impair several already compromised immune defence mechanisms and directly promote the growth of microbes (Boelaert et ah, 1996). 

Becker, S., Soukup, J.M., and Gallagher, J.E., Differential particulate air pollution induced oxidant stress in human granulocytes, monocytes and alveolar macrophages, Toxicol. In. Vitro. 16, 3, 209, 2002. 

NT072 Bagchi, D., E. A. Hassoun, M. Bagchi, and S. J. Stohs. Protective effects of free radical scavengers and antioxidants against smokeless tobacco extract (STE) induced oxidative stress in macrophage J774A.1. Cell cultures. Arch Environ Contam Toxicol 1995 29(3) 424-428. 

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