Macromolecular structures binding

Due to their macromolecular structure and structural heterogeneity, this type of CSPs possess several different binding sites. As a consequence, they usually have a very broad application range. 

Fortunately, once a parent macromolecular structure has been solved, new structures of the macromolecule complexed with different ligands can often be solved very quickly (within a few days in some cases (57)). These new structures are determined by cocrystallization of the ligand-macromolecule complex or by soaking protein crystals in a solution of the ligand and allowing the ligand to diffuse into the binding site. 

The reversible binding of aminoglycosides to macromolecular structures in blood and tissues is important for biological activity, pharmacokinetics, toxicity and excretion Binding equilibria of this type are difficult to analyse quantitatively, except for binding to plasma protein, for which several methods of determination (e.g. dialysis, ultrafiltration) exist Reported figures for serum binding... 

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